Research Papers:
microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10
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Abstract
Hongju Yang1,*, Qian Li2,*, Jie Niu3,*, Bai Li2, Dejun Jiang1 Zhihua Wan1, Qingmei Yang1, Fei Jiang1, Ping Wei1 and Song Bai1
1 The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
2 Human Genetics Center of Yunnan University, Kunming, Yunnan, China
3 Institute of Medicinal Biology Chinese Academy of Medical Science, Kunming, Yunnan, China
* These authors have contributed equally to this work
Correspondence to:
Song Bai, email:
Keywords: NAA10, miR-342-5p, miR-608, colon cancer
Received: June 03, 2015 Accepted: October 14, 2015 Published: December 04, 2015
Abstract
miRNAs have been shown to play pivotal roles in the establishment and progression of colon cancer, but their underlying mechanisms are not fully understood. N-acetyltransferase NAA10 participates in many cellular processes, including tumorigenesis. Here we showed that miR-342-5p and miR-608 suppressed the tumorigenesis of colon cancer cells in vitro and in vivo by targeting NAA10 mRNA for degradation. Overexpression of miR-342-5p or miR-608 decreased NAA10 mRNA and protein levels and thereby suppressed cell proliferation, migration, and cell-cycle progression, as well as promoted apoptosis in SW480 and SW620 cells. More importantly, miR-342-5p and miR-608 significantly decreased the tumorigenic capacity of SW480 and SW620 cells in a mouse xenograft model. We also observed an inverse correlation between the expression of NAA10 and that of both miRNAs. Our results implicate miR-342-5p and miR-608 in colon cancer development and unveil the underlying mechanism of this phenomenon, which involves NAA10.
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PII: 6458