Research Papers:
Genetic variants associated with gastrointestinal symptoms in Fabry disease
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Abstract
Maria Teresa Di Martino1,*, Francesca Scionti1,*, Simona Sestito2, Angela Nicoletti2, Mariamena Arbitrio3, Pietro Hiram Guzzi4, Valentina Talarico2, Federica Altomare2, Maria Teresa Sanseviero2, Giuseppe Agapito4, Antonio Pisani5, Eleonora Riccio5, Osvaldo Borrelli6, Daniela Concolino2,**, Licia Pensabene2,6,**
1Department of Experimental and Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, 88100 Italy
2Department of Medical and Surgical Sciences, Pediatric Unit, Magna Graecia University, Catanzaro, 88100 Italy
3ISN-CNR, Roccelletta di Borgia, Catanzaro, 88100 Italy
4Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, 88100 Italy
5Departement of Nephrology University Federico II, Naples, 80138 Italy
6Department of Pediatric Gastroenterology, Great Ormond Street Hospital for Sick Children, University College of London (UCL), London, WC1E 6BT, UK
*These authors have contributed equally to this work
**These authors have contributed equally to this work and share senior authorship
Correspondence to:
Licia Pensabene, email: [email protected]
Maria Teresa Di Martino, email: [email protected]
Keywords: Fabry disease, gastrointestinal symptoms, bile acids, DMET, genetic polymorphisms
Received: June 08, 2016 Accepted: October 29, 2016 Published: November 05, 2016
ABSTRACT
Gastrointestinal symptoms (GIS) are often among the earliest presenting events in Fabry disease (FD), an X-linked lysosomal disorder caused by the deficiency of α-galactosidase A. Despite recent advances in clinical and molecular characterization of FD, the pathophysiology of the GIS is still poorly understood. To shed light either on differential clinical presentation or on intervariability of GIS in FD, we genotyped 1936 genetic markers across 231 genes that encode for drug-metabolizing enzymes and drug transport proteins in 49 FD patients, using the DMET Plus platform. All nine single nucleotide polymorphisms (SNPs) mapped within four genes showed statistically significant differences in genotype frequencies between FD patients who experienced GIS and patients without GIS: ABCB11 (odd ratio (OR) = 18.07, P = 0,0019; OR = 8.21, P = 0,0083; OR=8.21, P = 0,0083; OR = 8.21, P = 0,0083),SLCO1B1 (OR = 9.23, P = 0,0065; OR = 5.08, P = 0,0289; OR = 8.21, P = 0,0083), NR1I3 (OR = 5.40, P = 0,0191) and ABCC5 (OR = 14.44, P = 0,0060). This is the first study that investigates the relationships between genetic heterogeneity in drug absorption, distribution, metabolism and excretion (ADME) related genes and GIS in FD. Our findings provide a novel genetic variant framework which warrants further investigation for precision medicine in FD.
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