Research Papers:
MicroRNA613 inhibits cell growth migration and invasion of papillary thyroid carcinoma by regulating SphK2
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Wangwang Qiu1,*, Zhili Yang1,*, Youben Fan1, Qi Zheng1
1Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, P.R. China
*These authors contributed equally to this work
Correspondence to:
Qi Zheng, email: [email protected]
Keywords: papillary thyroid cancer, miR-613, SphK2, cell growth, invasion
Received: January 18, 2016 Accepted: April 26, 2016 Published: May 21, 2016
ABSTRACT
MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in carcinogenesis. In this study, we investigated the biological function and mechanism of miR-613 in the regulation of papillary thyroid cancer (PTC) development. We found that miR-613 was downregulated in PTC cell lines and tissues, and overexpression of miR-613 significantly suppressed PTC cell growth, migration and invasion in vitro and inhibited tumor growth in vivo. We identified the gene for sphingosine kinase 2 (SphK2) as a direct target of miR-613. Overexpression of miR-613 significantly repressed SphK2 expression by directly targeting its 3′-untranslated regions (3′-UTR) and restoration of SphK2 reversed the inhibitory effects of miR-613 on PTC cell growth and invasion. Taken together, our results indicated that miR-613 functions as a tumor suppressor in PTC and its suppressive effect is mediated by repressing SphK2 expression.