Oncotarget

Research Papers:

A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer

Jiali Cai, Bingxin Gu, Fengwen Cao and Shiyuan Liu _

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Oncotarget. 2016; 7:40047-40059. https://doi.org/10.18632/oncotarget.9482

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Abstract

Jiali Cai1,*, Bingxin Gu2,3,4,5,*, Fengwen Cao6, Shiyuan Liu1

1Department of Radiology, Changzheng Hospital, Second Military Medical University, Shanghai, China

2Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai, China

3Center for Biomedical Imaging, Fudan University, Shanghai, China

4Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China

5Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai, China

6School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China

*These authors have contributed equally to this work

Correspondence to:

Shiyuan Liu, e-mail: [email protected]

Keywords: molecular imaging, transferrin, MRI/NIRF, non-small cell lung cancer, liver metastasis

Received: December 15, 2015    Accepted: April 11, 2016    Published: May 19, 2016

ABSTRACT

To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis.


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