Numb contributes to renal fibrosis by promoting tubular epithelial cell cycle arrest at G2/M

Fengxin Zhu; Wei Liu; Tang Li; Jiao Wan; Jianwei Tian; Zhanmei Zhou; Hao Li; Youhua Liu; Fan Fan Hou; Jing Nie

doi:10.18632/oncotarget.8238

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Research Papers:

Numb contributes to renal fibrosis by promoting tubular epithelial cell cycle arrest at G2/M

Fengxin Zhu _, Wei Liu, Tang Li, Jiao Wan, Jianwei Tian, Zhanmei Zhou, Hao Li, Youhua Liu, Fan Fan Hou and Jing Nie

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Oncotarget. 2016; 7:25604-25619. https://doi.org/10.18632/oncotarget.8238

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Abstract

Fengxin Zhu1, Wei Liu2, Tang Li3, Jiao Wan1, Jianwei Tian1, Zhanmei Zhou1, Hao Li1, Youhua Liu1, Fan Fan Hou1, Jing Nie1

1State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China

2Department of Nephrology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong Geriatric Institute, Guangzhou, P.R. China

3The VIP Medical Center, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China

Correspondence to:

Jing Nie e-mail: [email protected]

Keywords: Numb, G2/M arrest, p53, proximal tubular cells, interstitial fibrosis

Received: November 30, 2015 Accepted: March 06, 2016 Published: March 21, 2016

ABSTRACT

Numb is a multifunctional protein involved in diverse cellular processes. However, the function of Numb in kidney remains unclear. Here, we reported that Numb is expressed in renal tubules and glomeruli in normal adult kidney. Numb expression was upregulated in fibrotic kidneys induced by unilateral ureteral obstruction (UUO) in mice as well as in human fibrotic kidney tissues. Numb overexpression in cultured proximal tubular cells increased the G2/M cell population and upregulated the expression of TGF-β1 and CTGF. Whereas, proximal tubule Numb knockout (PEPCK-Numb-KO) mice showed reduced G2/M arrest, decreased expression of TGF-β1 and CTGF, and attenuated fibrotic lesions due to either UUO or unilateral ischemia reperfusion nephropathy. Inhibiting p53 activity by pifithrin-` dramatically mitigated Numb-induced G2/M arrest, indicating that Numb potentiates G2/M arrest via stabilizing p53 protein. Together, these data suggest that Numb is a potential target for anti-fibrosis therapy.

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Research Papers:

Numb contributes to renal fibrosis by promoting tubular epithelial cell cycle arrest at G2/M

Abstract