Oncotarget

Research Papers:

Dormancy activation mechanism of tracheal stem cells

Xin Li, Jing-xian Xu, Xin-Shan Jia _, Wen-ya Li, Yi-chen Han, En-hua Wang and Fang Li

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Oncotarget. 2016; 7:23730-23739. https://doi.org/10.18632/oncotarget.8179

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Abstract

Xin Li1,2,3, Jing-xian Xu4, Xin-Shan Jia2,3, Wen-ya Li5, Yi-chen Han2,3, En-hua Wang2,3, Fang Li6

1Department of Physiology, College of Life Science and Biopharmaceutics of Shenyang Pharmaceutical University, Shenyang, China

2Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, China

3Department of Pathology, First Affiliated Hospital of China Medical University, Shenyang, China

4Department of Ophthalmology, The 4th Affiliated Hospital, Eye Institute, China Medical University, The Key Laboratory of Lens Research, Shenyang, China

5Department of Thoracic Surgery, The First Affiliated Hospital, China Medical University, Shenyang, China

6IVF Michigan, Bloomfield Hills, MI, USA

Correspondence to:

Xin-Shan Jia, e-mail: [email protected]

Keywords: methylation, stem cell, dormancy, Sox2 expression

Received: December 17, 2015     Accepted: March 02, 2016     Published: March 18, 2016

ABSTRACT

Accurate markers and molecular mechanisms of stem cell dormancy and activation are poorly understood. In this study, the anti-cancer drug, 5-fluorouracil, was used to selectively kill proliferating cells of human bronchial epithelial (HBE) cell line. This method can enrich and purify stem cell population. The dormant versus active status of stem cells was determined by phosphorylation of RNAp II Ser2. The surviving stem cells were cultured to form stem cell spheres expressing stem cell markers and transplanted into nude mice to form a teratoma. The results demonstrated the properties of stem cells and potential for multi-directional differentiation. Bisulfite sequencing polymerase chain reaction showed that demethylation of the Sox2 promoter by 5-FU resulted in Sox2 expression in the dormant stem cells. This study shows that the dormancy and activation of HBE stem cells is closely related to epigenetic modification.


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