Oncotarget

Research Papers: Immunology:

Compensatory roles of CD8+ T cells and plasmacytoid dendritic cells in gut immune regulation for reduced function of CD4+ Tregs

Young-In Kim, Bo-Ra Lee, Jae-Hee Cheon, Bo-Eun Kwon, Mi-Na Kweon, Hyun-Jeong Ko and Sun-Young Chang _

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Oncotarget. 2016; 7:10947-10961. https://doi.org/10.18632/oncotarget.7510

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Abstract

Young-In Kim1,*, Bo-Ra Lee2,*, Jae-Hee Cheon3,*, Bo-Eun Kwon2, Mi-Na Kweon4, Hyun-Jeong Ko2,** and Sun-Young Chang1,**

1 College of Pharmacy, Ajou University, Suwon, Korea

2 College of Pharmacy, Kangwon National University, Chuncheon, Korea

3 Department of Internal Medicine and Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea

4 Mucosal Immunology Laboratory, Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

* These authors have contributed equally to this work

** Co-senior author

Correspondence to:

Sun-Young Chang, email:

Hyun-Jeong Ko, email:

Keywords: gut, CD4+ Tregs, CD8+ T cells, plasmacytoid dendritic cells, IL-10, Immunology and Microbiology Section, Immune response, Immunity

Received: September 18, 2015 Accepted: February 05, 2016 Published: February 19, 2016

Abstract

CD4+ Tregs need to migrate from the mucosal periphery into the draining lymph node via CCR7 to exert their suppressive effects. In this study, we investigated whether CCR7 deficiency resulted in failure of immune suppression in 2% dextran sulfate sodium-induced colitis. Unexpectedly, intestinal inflammation was not exacerbated in the absence of CCR7. Expression of IL-10, a representative suppressive cytokine, was enhanced in CCR7KO CD8+ T cells. Colon CCR7KO CD8+ T cells reduced the activation of CD4+ T cells. Depletion of CD8+ T cells using anti-CD8 antibody exacerbated colitis in CCR7KO mice. Plasmacytoid dendritic cell numbers were also slightly increased during intestinal inflammation in the absence of CCR7, and the depletion of those cells exacerbated DSS-induced colitis in CCR7KO mice. These results suggest that CD8+ T cells and plasmacytoid dendritic cells have compensatory roles in immune regulation in the gut for impaired function of CD4+ Tregs.


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