Research Papers:
Comparative effectiveness of light emitting diodes LEDs and Lasers in near infrared photoimmunotherapy
PDF | Full Text | Supplementary Files | How to cite
Kazuhide Sato1, Rira Watanabe1, Hirofumi Hanaoka1, Takahito Nakajima1, Peter L. Choyke1, Hisataka Kobayashi1
1Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
Correspondence to:
Hisataka Kobayashi, e-mail: [email protected]
Keywords: near infrared photoimmunotherapy, light emitting diode (LED), light amplification by stimulated emission of radiation (Laser), epidermal growth factor receptor, super-enhanced permeability and retention (SUPR) effect
Received: December 03, 2015 Accepted: January 29, 2016 Published: February 13, 2016
ABSTRACT
Near infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with the toxicity induced by photosensitizers after exposure to near infrared (NIR) light. Herein we compare two NIR-light sources; light emitting diodes (LEDs) and Lasers, for their effectiveness in NIR-PIT.
A photosensitizer, IRDye-700DX, conjugated to panitumumab (pan-IR700), was incubated with EGFR-expressing A431 and MDA-MB-468-luc cells. NIR-light was provided by LEDs or Lasers at the same light dose. Laser-light produced more cytotoxicity and greater reductions in IR700-fluorescence intensity than LED-light. Laser-light also produced more cytotoxicity in vivo in both cell lines. Assessment of super-enhanced permeability and retention (SUPR) effects were stronger with Laser than LED.
These results suggest that Laser-light produced significantly more cytotoxic effects compared to LEDs. Although LED is less expensive, Laser-light produces superior results in NIR-PIT.