Oncotarget

Research Papers:

MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing PCDH10

Ying Qi, Hong Sain Ooi, Jun Wu, Jian Chen, Xiaoli Zhang, Sheng Tan, Qing Yu, Yuan-Yuan Li, Yani Kang, Hua Li, Zirui Xiong, Tao Zhu, Bingya Liu _, Zhifeng Shao and Xiaodong Zhao

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Oncotarget. 2016; 7:12693-12703. https://doi.org/10.18632/oncotarget.7281

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Abstract

Ying Qi1, Hong Sain Ooi2, Jun Wu3, Jian Chen1, Xiaoli Zhang1, Sheng Tan4, Qing Yu4, Yuan-Yuan Li5,6, Yani Kang1, Hua Li1, Zirui Xiong4, Tao Zhu4, Bingya Liu1,7, Zhifeng Shao1, Xiaodong Zhao1

1School of Biomedical Engineering, Bio-ID Research Center, State Key Laboratory for Oncogenes and Related Genes, Shanghai Jiao Tong University, Shanghai, China

2Department of Biomedicine, Aarhus University, Aarhus, Denmark

3Department of Automation, Shanghai Jiao Tong University, Shanghai, China

4Laboratory of Molecular Tumor Pathology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China

5Shanghai Center for Bioinformatics Technology, Shanghai, China

6Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China

7Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Correspondence to:

Bingya Liu, e-mail: [email protected]

Xiaodong Zhao, e-mail: [email protected]

Keywords: EZH2, RIP-seq, MALAT1, gastric cancer, transcriptional silencing

Received: September 29, 2015     Accepted: January 23, 2016     Published: February 09, 2016

ABSTRACT

EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation and progression, in part through transcriptional silencing of tumor suppressor genes. A number of noncoding RNAs (ncRNAs) recruit EZH2 to specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) to profile EZH2-associated transcripts in human gastric cancer cell lines. We identified 8,256 transcripts, including both noncoding and coding transcripts, some of which were derived from cancer-related loci. In particular, we found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10, and promotes gastric cellular migration and invasion. Our work thus provides a global view of the EZH2-associated transcriptome and offers new insight into the function of EZH2 in gastric tumorigenesis.


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