Oncotarget

Research Papers:

Inhibiting cell migration and cell invasion by silencing the transcription factor ETS-1 in human bladder cancer

Li Liu, Yuchen Liu, Xintao Zhang, Mingwei Chen, Hanwei Wu, Muqi Lin, Yonghao Zhan, Chengle Zhuang, Junhao Lin, Jianfa Li, Wen Xu, Xing Fu, Qiaoxia Zhang, Xiaojuan Sun, Guoping Zhao and Weiren Huang _

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Oncotarget. 2016; 7:25125-25134. https://doi.org/10.18632/oncotarget.7192

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Abstract

Li Liu1,2,4,*, Yuchen Liu1,*, Xintao Zhang1,*, Mingwei Chen1, Hanwei Wu1, Muqi Lin1, Yonghao Zhan1,4, Chengle Zhuang1,4, Junhao Lin1,4, Jianfa Li1,4, Wen Xu1, Xing Fu1, Qiaoxia Zhang1, Xiaojuan Sun1, Guoping Zhao1,5, Weiren Huang1,3

1Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China

2Urology Department, Qingyuan People’s Hospital, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan, China

3Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China

4Shantou University Medical College, Shantou, China

5Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China

*These authors contributed equally to this work

Correspondence to:

Weiren Huang, email: [email protected]

Keywords: bladder cancer, ETS-1, oncogene, cell migration, cell invasion

Received: August 21, 2015     Accepted: January 20, 2016     Published: February 04, 2016

ABSTRACT

As one of the members of the ETS gene family, the transcription factor v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS-1) plays key role in the regulation of physiological processes in normal cells and tumors. In this study, we aimed to investigate the relationship between the transcription factor ETS-1 and malignant phenotypes of bladder cancer. We demonstrated that ETS-1 was up-regulated in human bladder cancer tissue compared to paired normal bladder tissue. In order to evaluate the functional role of ETS-1 in human bladder cancer, vectors expressing ETS-1 shRNA and ETS-1 protein were constructed in vitro and transfected into the human bladder cancer T24 and 5637 cells. Our results showed that the transcription factor ETS-1 could promote cell migration and cell invasion in human bladder cancer, without affecting cell proliferation and apoptosis. In conclusion, ETS-1 plays oncogenic roles through inducing cell migration and invasion in human bladder cancer, and it can be used as a therapeutic target for treating human bladder cancer.


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