Oncotarget

Research Papers:

Characterization of the novel tumor-suppressor gene CCDC67 in papillary thyroid carcinoma

De-Tao Yin _, Jianhui Xu, Mengyuan Lei, Hongqiang Li, Yongfei Wang, Zhen Liu, Yubing Zhou and Mingzhao Xing

PDF  |  HTML  |  How to cite

Oncotarget. 2016; 7:5830-5841. https://doi.org/10.18632/oncotarget.6709

Metrics: PDF 2102 views  |   HTML 2480 views  |   ?  


Abstract

De-Tao Yin1,2,*, Jianhui Xu1,2,*, Mengyuan Lei1,2, Hongqiang Li1,2, Yongfei Wang1,2, Zhen Liu1,2, Yubing Zhou3, Mingzhao Xing4

1Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P. R. China

2Key Discipline Laboratory of Clinical Medicine Henan, Zhengzhou 450052, P. R. China

3Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, P. R. China

4Division of Endocrinology and Metabolism, the Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA

*These authors contributed equally to this work

Correspondence to:

De-tao Yin, e-mail: [email protected]

Mingzhao Xing, e-mail: [email protected]

Keywords: thyroid cancer, CCDC67, tumor suppressor, prognostic marker, molecular pathogenesis

Received: September 01, 2015     Accepted: November 25, 2015     Published: December 21, 2015

ABSTRACT

Background: Some studies showed an association of coiled-coil domain-containing (CCDC) genes with cancers. Our previous limited data specifically suggested a possible pathogenic role of CCDC67 in papillary thyroid cancer (PTC), but this has not been firmly established. The present study was to further investigate and establish this role of CCDC67 in PTC.

Results: The expression of CCDC67, both at mRNA and protein levels, was sharply down-regulated in PTC compared with normal thyroid tissues. Lower CCDC67 expression was significantly associated with aggressive tumor behaviors, such as advanced tumor stages and lymph node metastasis, as well as BRAF mutation. Introduced expression of CCDC67 in TPC-1 cells robustly inhibited cell proliferation, colony formation and migration, induced G1 phase cell cycle arrest, and increased cell apoptosis.

Methods: Primary PTC tumors and matched normal thyroid tissues were obtained from 200 unselected patients at the initial surgery for detection of CCDC67 mRNA and protein by RT-PCR and Western blotting analyses, respectively. Genomic DNA sequencing was performed to detect BRAF mutation in PTC tumors. Clinicopathological data were retrospectively reviewed for correlation analyses. PTC cell line TPC-1 with stable transfection of CCDC67 was used to investigate the functions of CCDC67.

Conclusions: This large study demonstrates down-regulation of CCDC67 in PTC, an inverse relationship between CCDC67 expression and PTC aggressiveness and BRAF mutation, and a robust inhibitory effect of CCDC67 on PTC cellular activities. These results are consistent with CCDC67 being a novel and impaired tumor suppressor gene in PTC, providing important prognostic and therapeutic implications for this cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 6709