Research Papers:
Dietary NiCl2 causes G2/M cell cycle arrest in the broiler’s kidney
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Abstract
Hongrui Guo1, Hengmin Cui1,2, Xi Peng1,2, Jing Fang1,2, Zhicai Zuo1,2, Junliang Deng1,2, Xun Wang1,2, Bangyuan Wu1, Kejie Chen1 and Jie Deng1
1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya’an, China
2 College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China
Correspondence to:
Hengmin Cui, email:
Keywords: NiCl2, G2/M cell cycle arrest, mRNA expression, protein expression, kidney
Received: June 29, 2015 Accepted: September 14, 2015 Published: September 30, 2015
Abstract
Here we showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase. The G2/M cell cycle arrest was accompanied by up-regulation of phosphorylated ataxia telangiectasia mutated (p-ATM), p53, p-Chk1, p-Chk2, p21 protein expression and ATM, p53, p21, Chk1, Chk2 mRNA expression, and down-regulation of p-cdc25C, cdc2, cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and the cdc25, cdc2, cyclinB, PCNA mRNA expression.
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