Oncotarget

Research Papers: Gerotarget (Focus on Aging):

The effects of graded levels of calorie restriction: III. Impact of short term calorie and protein restriction on mean daily body temperature and torpor use in the C57BL/6 mouse

Sharon E. Mitchell, Camille Delville, Penelope Konstantopedos, Davina Derous, Cara L. Green, Luonan Chen, Jing-Dong J. Han, Yingchun Wang, Daniel E.L. Promislow, Alex Douglas, David Lusseau and John R. Speakman _

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Oncotarget. 2015; 6:18314-18337. https://doi.org/10.18632/oncotarget.4506

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Abstract

Sharon E. Mitchell1, Camille Delville1, Penelope Konstantopedos1, Davina Derous1, Cara L. Green1, Luonan Chen2, Jing-Dong J. Han3, Yingchun Wang4, Daniel E.L. Promislow5, Alex Douglas1, David Lusseau1, John R. Speakman1,4

1Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK

2Key laboratory of Systems Biology, Innovation Center for Cell Signaling Network, Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, China

3Chinese Academy of Sciences Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

4State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang, Beijing, China

5Department of Pathology, University of Washington at Seattle, Seattle, Washington, USA

Correspondence to:

John R. Speakman, e-mail: [email protected]

Keywords: Gerotarget, dietary restriction, protein restriction, calorie restriction, body temperature, torpor

Received: May 01, 2015     Accepted: July 13, 2015     Published: July 22, 2015

ABSTRACT

A commonly observed response in mammals to calorie restriction (CR) is reduced body temperature (Tb). We explored how the Tb of male C57BL/6 mice responded to graded CR (10 to 40%), compared to the response to equivalent levels of protein restriction (PR) over 3 months. Under CR there was a dynamic change in daily Tb over the first 30–35 days, which stabilized thereafter until day 70 after which a further decline was noted. The time to reach stability was dependent on restriction level. Body mass negatively correlated with Tb under ad libitum feeding and positively correlated under CR. The average Tb over the last 20 days was significantly related to the levels of body fat, structural tissue, leptin and insulin-like growth factor-1. Some mice, particularly those under higher levels of CR, showed periods of daily torpor later in the restriction period. None of the changes in Tb under CR were recapitulated by equivalent levels of PR. We conclude that changes in Tb under CR are a response only to the shortfall in calorie intake. The linear relationship between average Tb and the level of restriction supports the idea that Tb changes are an integral aspect of the lifespan effect.


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