Oncotarget

Research Papers:

Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs

Qi Zhang, Luxia Jiang, Jing Yu, Limin Tian, Tiankang Guo, Baoshan Di, Jinxing Quan, Jing Feng and Jing Liu _

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Oncotarget. 2019; 10:4083-4090. https://doi.org/10.18632/oncotarget.26937

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Abstract

Qi Zhang1,2,*, Luxia Jiang3,*, Jing Yu4, Limin Tian1, Tiankang Guo1, Baoshan Di5, Jinxing Quan1, Jing Feng1 and Jing Liu1

1 Department of Endocrinology, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China

2 School of Basic Medical Sciences, Lanzhou University, Lanzhou City 730000, Gansu Province, China

3 Intensive Care Unit, Lanzhou University Second Hospital, Lanzhou City 730000, Gansu Province, China

4 Gansu University of Chinese Medicine, Lanzhou City 730000, Gansu Province, China

5 Emergency Department, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China

* These authors contributed equally to this work

Correspondence to:

Jing Liu,email: [email protected]

Keywords: human liver sinusoidal endothelial cells; integrin αvβ3; laminin

Received: September 14, 2017     Accepted: March 24, 2018     Published: June 25, 2019

ABSTRACT

Aims/Introduction

To investigate the roles of reactive oxygen species (ROS) and integrin αvβ3 in palmitate-induced laminin expression of human liver sinusoidal endothelial cells (HLSECs).

Results

The protein expression of integrin αv, integrin β3 and laminin are increased by palmitate in HLSECs in a time- and dose-dependent manner. NAC, the ROS inhibitor, significantly inhibited the up-regulation of protein expression of integrin αv, integrin β3 and laminin by palmitate (P < 0.05). Palmitate markedly enhanced ROS formation (P < 0.05), which was not inhibited by LM609, the antibody of integrin αvβ3. Palmitate significantly increased laminin synthesis (P < 0.05), which was attenuated by LM609 and NAC (P < 0.05).

Materials and Methods

HLSECs were treated with palmitate in the presence or absence of LM609 (10 μg/ml) or N-acetylcysteine (NAC) (2 mM). Expression of integrin αv, integrin β3 and laminin were measured by RT-PCR and Western blot. Immunocytochemistry were used for examining laminin expression. The generation of ROS was measured using the fluorescent signal 2',7' dichloro-fluorescein diacetate (DCFH-DA).

Conclusions

The results suggested that palmitate increases laminin expression through ROS/integrin αv/β3 pathway.


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