Oncotarget

Research Papers:

Neat1 in hematopoietic stem cells

Noam Fallik, Yael Bar-Lavan, Yariv Greenshpan, Oron Goldstein, Markus Grosch, Micha Drukker and Roi Gazit _

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Oncotarget. 2017; 8:109575-109586. https://doi.org/10.18632/oncotarget.22729

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Abstract

Noam Fallik1, Yael Bar-Lavan1,2,3, Yariv Greenshpan1,2, Oron Goldstein1,2,3, Markus Grosch4, Micha Drukker4 and Roi Gazit1,2,3

1The Shraga Segal Department for Microbiology Immunology and Genetics, Faculty of Health Sciences, The Ben-Gurion University of the Negev, Be’er Sheva, Israel

2National Institute for Biotechnology in the Negev, The Ben-Gurion University of the Negev, Be’er Sheva, Israel

3Center for Regenerative Medicine and Stem Cells, The Ben-Gurion University of the Negev, Be’er Sheva, Israel

4Institute of Stem Cell Research, German Research Center for Environmental Health, Helmholtz Center Munich, Neuherberg, Germany

Correspondence to:

Roi Gazit, email: [email protected]

Keywords: Neat1; hematopoietic stem cells; paraspeckles; hematopoiesis; long non-coding RNAs

Received: September 11, 2017    Accepted: November 10, 2017    Published: November 30, 2017

ABSTRACT

Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. Neat1 is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected Neat1 expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms. Neat1 is highly expressed in stem and progenitor cells, yet it shows significant reduction in granulocytes. Microscopically, Neat1 is detected as sharp nuclear foci. Paraspeckle proteins NONO and PSPC1 are detected as aggregated nuclear foci in fresh primary hematopoietic cells, and in cultured cells. Induction of differentiation in vitro was found to enhance Neat1 expression. Taken together, our data demonstrate for the first time the expression of Neat1 and paraspeckles formation in HSCs and along hematopoiesis.


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