Oncotarget

Research Papers:

Detection of HPV related oropharyngeal cancer in oral rinse specimens

Matthew Rosenthal, Bin Huang, Nora Katabi, Jocelyn Migliacci, Robert Bryant, Samuel Kaplan, Timothy Blackwell, Snehal Patel, Liying Yang, Zhiheng Pei, Yi-Wei Tang and Ian Ganly _

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Oncotarget. 2017; 8:109393-109401. https://doi.org/10.18632/oncotarget.22682

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Abstract

Matthew Rosenthal1, Bin Huang2,7, Nora Katabi3, Jocelyn Migliacci1, Robert Bryant1, Samuel Kaplan2, Timothy Blackwell1, Snehal Patel1, Liying Yang4, Zhiheng Pei4,5,6, Yi-Wei Tang2,8 and Ian Ganly1

1Head and Neck Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA

2Clinical Microbiology Service, Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA

3Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA

4Department of Medicine, New York University School of Medicine, New York, NY, USA

5Department of Pathology, New York University School of Medicine, New York, NY, USA

6Department of Veterans Affairs New York Harbor Healthcare System, New York, NY, USA

7Department of Laboratory Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

8Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY, USA

Correspondence to:

Ian Ganly, email: [email protected]

Keywords: human papillomavirus; oral rinse; oropharynx cancer; oral cavity cancer; screening test

Received: July 03, 2017     Accepted: November 13, 2017     Published: November 25, 2017

ABSTRACT

Background: The majority of patients diagnosed with oropharyngeal squamous cell cancer (OPSCC) are due to HPV infection. At present, there are no reliable tests for screening HPV in patients with OPSCC. The objective of this study was to assess the Cobas® HPV Test on oral rinse specimens as an early, non-invasive tool for HPV-related OPSCC.

Methods: Oral rinse specimens were collected from 187 patients (45 with OPSCC, 61 with oral cavity SCC (OCSCC) and 81 control patients who had benign or malignant thyroid nodules) treated at MSKCC. The Cobas® HPV Test was used to detect 14 high-risk HPV types in these samples. Performance of the HPV Test was correlated with p16 tumor immunohistochemistry as gold standard.

Results: 91.1% of the oropharynx cancer patients had p16 positive tumors compared to 3.3% of oral cavity cancer. Of the 81 control patients, 79 (97.5%) had no HPV in their oral rinse giving a specificity of the HPV test of 98%. For the combined oral cavity oropharynx cancer cohort, the sensitivity, specificity, positive predictive value and negative predictive value of the HPV Test were 79.1%, 90.5%, 85.0% and 86.4% respectively when p16 immunohistochemistry was used as the reference.

Conclusion: The Cobas® HPV Test on oral rinse is a highly specific and potentially sensitive test for oropharyngeal cancer and may be a potentially useful screening test for early oropharyngeal cancer.

Impact: We describe an oral rinse test for the detection of HPV related oropharyngeal cancer.


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