Activation of AMPK by OSU53 protects spinal cord neurons from oxidative stress

Jun Xu; Liang Wu; Yiming Zhang; Huijie Gu; Zhongyue Huang; Kaifeng Zhou; Xiaofan Yin

doi:10.18632/oncotarget.22055

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Learn about our FREE

Post-Publication Promotion Services

50% on all publication fees until the end of 2024.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Activation of AMPK by OSU53 protects spinal cord neurons from oxidative stress

Jun Xu, Liang Wu, Yiming Zhang, Huijie Gu, Zhongyue Huang, Kaifeng Zhou and Xiaofan Yin _

PDF | HTML | How to cite

Oncotarget. 2017; 8:112477-112486. https://doi.org/10.18632/oncotarget.22055

Metrics: PDF 1404 views | HTML 2321 views | ?

Abstract

Jun Xu1, Liang Wu1,*, Yiming Zhang1, Huijie Gu1, Zhongyue Huang1, Kaifeng Zhou1 and Xiaofan Yin1

1Department of Orthopedics, Minhang Hospital, Fudan University, Shanghai, China

*Co-First author

Correspondence to:

Xiaofan Yin, email: [email protected]

Keywords: spinal cord injury, oxidative stress, AMPK and OSU53

Received: July 27, 2017 Accepted: August 28, 2017 Published: October 23, 2017

ABSTRACT

The present study tested the potential effect of OSU53, a novel AMPK activator, against hydrogen peroxide (H2O2)-induced spinal cord neuron damages. Treatment with OSU53 attenuated H2O2-induced death and apoptosis of primary murine spinal cord neurons. OSU53 activated AMPK signaling, which is required for its actions in spinal cord neurons. The AMPK inhibitor Compound C or AMPKα1 siRNA almost abolished OSU53-mediated neuroprotection against H2O2. On the other hand, sustained-activation of AMPK by introducing the constitutive-active AMPKα1 mimicked OSU53’s actions, and protected spinal cord neurons from oxidative stress. OSU53 significantly attenuated H2O2-induced reactive oxygen species production, lipid peroxidation and DNA damages in spinal cord neurons. Additionally, OSU53 increased NADPH content and heme oxygenase-1 mRNA expression in H2O2-treated spinal cord neurons. Together, we indicate that targeted-activation of AMPK by OSU53 protects spinal cord neurons from oxidative stress.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 22055

Publication Alerts

Post-Publication Promotion

Publication Discount

Research Papers:

Activation of AMPK by OSU53 protects spinal cord neurons from oxidative stress

Abstract