Oncotarget

Research Papers:

This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.

Reduced m6A mRNA methylation is correlated with the progression of human cervical cancer

Xiuli Wang, Zenghui Li, Beihua Kong _, Chen Song, Jianglin Cong, Jianqing Hou and Shaoguang Wang

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2017; 8:98918-98930. https://doi.org/10.18632/oncotarget.22041

Metrics: PDF 2727 views  |   HTML 5990 views  |   ?  


Abstract

Xiuli Wang1,2,*, Zenghui Li2,*, Beihua Kong1, Chen Song3, Jianglin Cong2, Jianqing Hou2 and Shaoguang Wang2

1Department of Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China

2The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, P.R. China

3Department of Gynecology, Zhucheng Maternal and Child Health Hospital, Zhucheng, Shandong 262200, P.R. China

*These authors have contributed equally to this work

Correspondence to:

Beihua Kong, email: [email protected]

Keywords: m6A RNA methylation; cervical cancer; cancer

Received: May 04, 2017     Accepted: October 02, 2017     Published: October 24, 2017

ABSTRACT

The m6A mRNA methylation involves in mRNA splicing, degradation and translation. Recent studies have revealed that reduced m6A mRNA methylation might promote cancer development. However, the role of m6A mRNA methylation in cervical cancer development remains unknown. Therefore, we investigated the role of m6A methylation in cervical cancer in the current study. We first evaluated the m6A mRNA methylation level in 286 pairs of cervical cancer samples and their adjacent normal tissues by dot blot assay. Then the role of m6A on patient survival rates and cervical cancer progression were assessed. The m6A level was significantly reduced in the cervical cancer when comparing with the adjacent normal tissue. The m6A level reduction was significantly correlated with the FIGO stage, tumor size, differentiation, lymph invasion and cancer recurrence. It was also shown to be an independent prognostic indicator of disease-free survival and overall survival for patients with cervical cancer. Reducing m6A level via manipulating the m6A regulators expression promoted cervical cancer cell proliferation. And increasing m6A level significantly suppressed tumor development both in vitro and in vivo. Our results showed that the reduced m6A level is tightly associated with cervical cancer development and m6A mRNA methylation might be a potential therapeutic target in cervical cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 22041