Oncotarget

Research Papers:

Role of HIF-1a in regulating autophagic cell survival during cerebral ischemia reperfusion in rats

Yongqing Guo _

PDF  |  HTML  |  How to cite

Oncotarget. 2017; 8:98482-98494. https://doi.org/10.18632/oncotarget.21445

Metrics: PDF 1875 views  |   HTML 3958 views  |   ?  


Abstract

Yongqing Guo1

1Department of Anesthesiology, Shanxi Provincial People’s Hospital, Taiyuan 030012, China

Correspondence to:

Yongqing Guo, email: [email protected]

Keywords: autophagy; apoptosis; cerebral ischemia reperfusion; HIF-1a

Received: June 26, 2017     Accepted: August 08, 2017     Published: October 01, 2017

ABSTRACT

Hypoxia-inducible factor-1a (HIF-1a) plays a beneficial role during cerebral ischemia reperfusion (IR), but the underlying molecular mechanisms are not completely understood. Here, we aimed to investigate the effects and molecular regulation of HIF-1a on brain cell apoptosis and autophagy during IR. We found that augmentation of HIF-1a in re-perfused hematopoietic cells significantly reduced brain damage, alleviated brain edema and improved neural function during IR, seemingly through two HIF-1a target genes BNIP3 and NIX, which were critical regulators for cell apoptosis and autophagic cell survival. in vitro, HIF-1a induced up-regulation of BNIP3 and NIX in human cortical neuron cells, HCN-1A. Inhibition of BNIP3 and NIX significantly attenuated HIF-1a-suppressed cell apoptosis and HIF-1a-induced cell autophagy. Together, these data suggest that HIF-1a may ameliorate brain damages during IR through BNIP3 and NIX -dependent augmentation of autophagic cell survival and reduction in cell apoptosis.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 21445