MicroRNA-29a increased the intestinal membrane permeability of colonic epithelial cells in irritable bowel syndrome rats

Guanqun Chao; Yingying Wang; Shuo Zhang; Weilin Yang; Zheying Ni; Xuliang Zheng

doi:10.18632/oncotarget.20687

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This article has been corrected. Correction in: Oncotarget. 2018; 9:15816.

MicroRNA-29a increased the intestinal membrane permeability of colonic epithelial cells in irritable bowel syndrome rats

Guanqun Chao, Yingying Wang, Shuo Zhang _, Weilin Yang, Zheying Ni and Xuliang Zheng

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Oncotarget. 2017; 8:85828-85837. https://doi.org/10.18632/oncotarget.20687

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Abstract

Guanqun Chao1,*, Yingying Wang2,*, Shuo Zhang2,*, Weilin Yang2, Zheying Ni2 and Xuliang Zheng2

1Department of Family Medicine, Sir Run Run Shaw Hospital, Zhejiang University, China

2Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, China

*These authors have contributed equally to this work

Correspondence to:

Shuo Zhang, email: [email protected]

Keywords: diarrhea-predominant irritable bowel syndrome (IBS-D), microRNA-29a, intestinal membrane permeability, aquaporins (AQPs)

Abbreviations: IBS-D: diarrhea-predominant irritable bowel syndrome, miR-29a: microRNA-29a, AQPs: aquaporins, miRNAs: MicroRNAs, LDH: lactate dehydrogenase

Received: May 17, 2017 Accepted: July 26, 2017 Published: September 06, 2017

ABSTRACT

Background: The whole pathogenesis of diarrhea-predominant irritable bowel syndrome(IBS-D) is poorly understood. Our goal was to evaluate the expression change of microRNA-29a(miR-29a) in colonic epithelial cells in IBS rats and clarify the mechanism of miR-29a increasing the intestinal membrane permeability through aquaporins(AQPs).

Methods: The IBS-D rats models were induced by rectal distention pressure combining with extremities constraint. The colonic epithelial cells were divided into four groups. A: normal group. B: IBS-D control group. C: IBS-D +miR-29a NC. D: IBS-D + miR-29a antagomir. The expression of miR-29a, the concentration of the K⁺ and Lactate Dehydrogenase(LDH) and the expression of AQPs were detected.

Results: The miR-29a expression increased in IBS-D control group(2.090±0.022) compared with the control group(1.00±0.031) (P<0.001) while it decreased in IBS-D+miR-29a antagomir group(1.403±0.042) compared with IBS-D control group(P<0.001). The K⁺ decreased in IBS-D control group(1.305±0.289) compared with the control group(2.171±0.204)(P<0.05) while it increased in IBS-D+miR-29a antagomir group(1.813±0.102)(P<0.05) compared with IBS-D control group. The LDH increased in IBS-D control group(4153.440±177.365) compared with the control group(1434.573±96.111)(P<0.001) while it decreased in IBS-D+miR-29a antagomir group(2700.473±275.414) compared with IBS-D control group (P<0.01). The expression of AQP1, AQP3 and AQP8 decreased in IBS-D control group(0.132±0.010,0.110±0.005,0.108±0.007) compared with the control group (P<0.001) while it increased in IBS-D+miR-29a antagomir group(0.197±0.005,0.182±0.011,0.194±0.003) compared with IBS-D control group(P<0.001). The IBS-D+miR-29a negative control(NC) group, a comparison with IBS-D+miR-29a antagomir group, each date showed the similar trend to the IBS-D control group.

Conclusions: MiR-29a increased the intestinal membrane permeability of colonic epithelial cells by reducing the AQPs expression in IBS-D rats.

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MicroRNA-29a increased the intestinal membrane permeability of colonic epithelial cells in irritable bowel syndrome rats

Abstract