Oncotarget

Research Papers:

Sperm-carried RNAs play critical roles in mouse embryonic development

Lei Guo, Shi-Bin Chao, Lu Xiao, Zhen-Bo Wang, Tie-Gang Meng, Yuan-Yuan Li, Zhi-Ming Han, Ying-Chun Ouyang, Yi Hou, Qing-Yuan Sun and Xiang-Hong Ou _

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Oncotarget. 2017; 8:67394-67405. https://doi.org/10.18632/oncotarget.18672

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Abstract

Lei Guo1,*, Shi-Bin Chao2,3,*, Lu Xiao4, Zhen-Bo Wang2,5, Tie-Gang Meng2,4, Yuan-Yuan Li2, Zhi-Ming Han2, Ying-Chun Ouyang2, Yi Hou2, Qing-Yuan Sun2,5 and Xiang-Hong Ou1

1Center for Reproductive Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, PR China

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China

3The ART Center, Jiujiang Maternal and Child Health Care Hospital, Jiangxi 332000, PR China

4Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China

5College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China

*These authors have contributed equally to this work

Correspondence to:

Xiang-Hong Ou, email: [email protected]

Qing-Yuan Sun, email: [email protected]

Keywords: sperm RNA, RNase, intracytoplasmic sperm injection, blastocyst formation, offspring

Received: March 28, 2017     Accepted: May 22, 2017     Published: June 27, 2017

ABSTRACT

Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs and the sperm delivers some RNAs to the oocyte at fertilization. However, the functions of the RNAs carried to the oocyte by sperm at fertilization in embryonic development remains a mystery. In this study, the mature spermatozoa were treated with lysolecithin, pronase and RNases (RNase A and RNase H) to remove the sperm-carried RNAs, and then injected into normal mature oocyte. The results showed that after the treatment, the content of the sperm RNAs was decreased by about 90%. The blastocyst formation rate and the live birth rate of the embryos from intracytoplasmic sperm injection (ICSI) using the treated sperm were significantly decreased (P<0.01), while these effects were partially rescued by injecting total wide-type sperm RNAs. The reproductive capacity of offspring (F0) in sperm-treated group was similar with that in control group (P>0.05), but the body weight of F1 mice from sperm-treated group was lower than that in control group after two weeks of birth (P<0.05). These results demonstrated that the sperm-carried RNAs have important roles in embryonic development.


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