Oncotarget

Research Papers:

Rap2b siRNA significantly enhances the anticancer therapeutic efficacy of Adriamycin in a gold nanoshell-based drug/gene co-delivery system

Li Ding, Ruonan Sun and Xinyue Zhang _

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Oncotarget. 2017; 8:21200-21211. https://doi.org/10.18632/oncotarget.15508

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Abstract

Li Ding1,*, Ruonan Sun1,*, Xinyue Zhang1,2,3,4,5

1College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, Jiangsu 225009, China

2Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China

3Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China

4Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu 225009, China

5Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, Jiangsu 225009, China

*These authors have contributed equally to this work

Correspondence to:

Xinyue Zhang, email: [email protected]

Keywords: Rap2b, siRNA, Adriamycin, gold nanoshells, cancer therapy

Received: December 14, 2016     Accepted: February 07, 2017     Published: February 19, 2017

ABSTRACT

Rap2b is a novel p53 target we have identified recently. Knockdown of Rap2b sensitizes HCT116 cells to adriamycin-induced apoptosis, indicating that Rap2b promotes adriamycin resistance in cancer cells. In the present study, we designed a nanostructure-based drug/gene delivery system to evaluate the potential of Rap2b siRNA as a therapeutic agent against human cancers. Specifically, after co-incubated with HCT116 cells, adriamycin- and Rap2b siRNA-loaded gold nanoshells were internalized. Subsequent laser irradiation promoted release of adriamycin and Rap2b siRNA from the nanoparticles. The laser-induced release of Rap2b siRNA decreased cellular expression of Rap2b and significantly enhanced the anticancer therapeutic efficacy of adriamycin in vitro and in vivo. In addition, laser irradiation of the nanoparticles might exert an additional thermal killing effect on cancer cells and further improved the anticancer efficacy of adriamycin. In summary, Rap2b siRNA is a potential enhancing agent for adriamycin-based anticancer therapeutics and the gold nanoshell-based drug/gene delivery system carrying both adriamycin and Rap2b siRNA provides a promising anticancer therapeutic strategy.


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