Research Papers:
Reciprocal control of lncRNA-BCAT1 and β-catenin pathway reveals lncRNA-BCAT1 long non-coding RNA acts as a tumor suppressor in colorectal cancer
Metrics: PDF 1979 views | HTML 2027 views | ?
Abstract
Fei Xie1,*, Xudong Xiang2,*, Qionglin Huang1,*, Pengzhan Ran1, Yuncang Yuan1, Qian Li1, Guoxiang Qi1, Xiaopeng Guo1, Chunjie Xiao1,**, Shangyong Zheng1,**
1School of Medicine, Yunnan University, Kunming, Yunnan 650091, P.R. China
2Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China
*These authors have contributed equally to this work
**These two authors are co-corresponding authors
Correspondence to:
Chunjie Xiao, email: [email protected]
Shangyong Zheng, email: [email protected]
Keywords: long non-coding RNA, lncRNA-BCAT1, β-catenin, tumor suppressor, colorectal cancer
Received: September 23, 2016 Accepted: December 27, 2016 Published: February 17, 2017
ABSTRACT
β-catenin plays a major role in tumor development and progression. The present study found that β-catenin was upregulated in 30 samples of colorectal cancer (CRC) tissue as compared to adjacent non-tumor tissues. Analysis of long non-coding RNA (lncRNA) expression profiles using the GSE18560 and GSE44097 datasets, which were generated via the Affymetrix plus 2.0 microarray platform and downloaded from the GEO database, revealed 20 differentially expressed lncRNAs following β-catenin knockdown. We focused on AK091631, a novel lncRNA, which we named lncRNA-β-catenin associated transcript 1 (LncRNA-BCAT1). lncRNA-BCAT1 expression was decreased in CRC tissues, and was negatively associated with β-catenin in both CRC tissues and cell lines. lncRNA-BCAT1 overexpression suppressed CRC cell growth and invasion by downregulating cyclin D1, c-Myc, and MMP-2. These results suggest that lncRNA-BCAT1 overexpression inhibits CRC cell growth and invasion via Wnt/β-catenin pathway blockade, and that lncRNA-BCAT1 is repressed by Wnt/β-catenin signaling. This evidence suggests that lncRNA-BCAT1 is a tumor suppressor and that lncRNA-BCAT1 may be an effective prognostic biomarker in CRC.
![Creative Commons License](/images/80x15.png)
PII: 15466