Research Papers:
Stromal fibroblasts present in breast carcinomas promote tumor growth and angiogenesis through adrenomedullin secretion
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Abstract
Zohra Benyahia1, Nadège Dussault1, Mylène Cayol1, Romain Sigaud1, Caroline Berenguer-Daizé1, Christine Delfino1, Asma Tounsi1, Stéphane Garcia2, Pierre-Marie Martin1, Kamel Mabrouk3, L’Houcine Ouafik1,4
1Aix Marseille Univ, The Institut National pour la Recherche Médicale, Centre de Recherche en Oncologie et Oncopharmacologie, UMR 911, 13005, Marseille, France
2Assistance Publique Hôpitaux de Marseille, Laboratoire d’Anatomie Pathologique, 13015, Marseille, France
3Aix Marseille Univ, CNRS, ICR, UMR 7273 CROPS, 13397, Marseille, France
4Assistance Publique Hôpitaux de Marseille, Service de Transfert d’Oncologie Biologique, 13015, Marseille, France
Correspondence to:
L’Houcine Ouafik email: [email protected]
Keywords: adrenomedullin, breast cancer, myofibroblasts, invasion, tumor growth
Received: July 20, 2016 Accepted: January 03, 2017 Published: February 02, 2017
ABSTRACT
Tumor- or cancer-associated fibroblasts (TAFs or CAFs) are active players in tumorigenesis and exhibit distinct angiogenic and tumorigenic properties. Adrenomedullin (AM), a multifunctional peptide plays an important role in angiogenesis and tumor growth through its receptors calcitonin receptor-like receptor/receptor activity modifying protein-2 and -3 (CLR/RAMP2 and CLR/RAMP3). We show that AM and AM receptors mRNAs are highly expressed in CAFs prepared from invasive breast carcinoma when compared to normal fibroblasts. Immunostaining demonstrates the presence of immunoreactive AM and AM receptors in the CAFs (n = 9). The proliferation of CAFs is decreased by anti-AM antibody (αAM) and anti-AM receptors antibody (αAMR) treatment, suggesting that AM may function as a potent autocrine/paracrine growth factor. Systemic administration of αAMR reduced neovascularization of in vivo Matrigel plugs containing CAFs as demonstrated by reduced numbers of the vessel structures, suggesting that AM is one of the CAFs-derived factors responsible for endothelial cell-like and pericytes recruitment to built a neovascularization. We show that MCF-7 admixed with CAFs generated tumors of greater volume significantly different from the MCF-7 xenografts in nude mice due in part to the induced angiogenesis. αAMR and AM22-52 therapies significantly suppressed the growth of CAFs/MCF-7 tumors. Histological examination of tumors treated with AM22-52 and aAMR showed evidence of disruption of tumor vasculature with depletion of vascular endothelial cells, induced apoptosis and decrease of tumor cell proliferation. Our findings highlight the importance of CAFs-derived AM pathway in growth of breast carcinoma and in neovascularization by supplying and amplifying signals that are essential for pathologic angiogenesis.
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PII: 14999