Oncotarget

Research Papers:

Talin-1 interaction network promotes hepatocellular carcinoma progression

Peng Chen, Xiaohu Zheng, Yonggang Zhou, Yechuan Xu, Lixin Zhu and Yeben Qian _

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Oncotarget. 2017; 8:13003-13014. https://doi.org/10.18632/oncotarget.14674

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Abstract

Peng Chen1,*, Xiaohu Zheng2,*, Yonggang Zhou2, Yechuan Xu1, Lixin Zhu3, Yeben Qian1

1Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China

2Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, China

3Center Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei, China

*These authors contributed equally to this work

Correspondence to:

Yeben Qian, email: [email protected]

Keywords: Talin-1, hepatocellular carcinoma, tumor growth and metastasis, ion transport, membrane depolarization

Received: October 09, 2016     Accepted: January 09, 2017     Published: January 16, 2017

ABSTRACT

Talin-1 is a known oncogene-associated protein. In this study, we set out to determine its role and mechanisms in hepatocellular carcinoma (HCC) progression. We found Talin-1 to be highly expressed in HCC cells relative to non-cancer liver epithelial cells and to promote tumor growth and metastasis. We used Whole Human Genome Oligo Microarray analysis with HCC cells and HCC cells in which Talin-1 was knocked down using shRNA to identify transcripts regulated by Talin-1. Of the 40,000 tested genes, 3099 were differentially expressed after Talin-1 knockdown; expression of 1924 genes was increased, while expression of 2175 was decreased. Gene ontology (GO) profiling indicated that Talin-1 promotes many HCC progression-related activities, including ion transport and membrane depolarization, cell growth, and cell adhesion. We also characterized the network of gene transcripts regulated by Talin-1 and their role in promoting HCC progression. Our findings confirm the role of Talin-1 in carcinogenesis and provided a set of novel therapeutic targets for the treatment of HCC.


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