Research Papers:
Talin-1 interaction network promotes hepatocellular carcinoma progression
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Abstract
Peng Chen1,*, Xiaohu Zheng2,*, Yonggang Zhou2, Yechuan Xu1, Lixin Zhu3, Yeben Qian1
1Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China
2Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, China
3Center Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei, China
*These authors contributed equally to this work
Correspondence to:
Yeben Qian, email: [email protected]
Keywords: Talin-1, hepatocellular carcinoma, tumor growth and metastasis, ion transport, membrane depolarization
Received: October 09, 2016 Accepted: January 09, 2017 Published: January 16, 2017
ABSTRACT
Talin-1 is a known oncogene-associated protein. In this study, we set out to determine its role and mechanisms in hepatocellular carcinoma (HCC) progression. We found Talin-1 to be highly expressed in HCC cells relative to non-cancer liver epithelial cells and to promote tumor growth and metastasis. We used Whole Human Genome Oligo Microarray analysis with HCC cells and HCC cells in which Talin-1 was knocked down using shRNA to identify transcripts regulated by Talin-1. Of the 40,000 tested genes, 3099 were differentially expressed after Talin-1 knockdown; expression of 1924 genes was increased, while expression of 2175 was decreased. Gene ontology (GO) profiling indicated that Talin-1 promotes many HCC progression-related activities, including ion transport and membrane depolarization, cell growth, and cell adhesion. We also characterized the network of gene transcripts regulated by Talin-1 and their role in promoting HCC progression. Our findings confirm the role of Talin-1 in carcinogenesis and provided a set of novel therapeutic targets for the treatment of HCC.
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PII: 14674