Research Papers:
Kinetic changes of intestinal microbiota in the course of intestinal sensitization
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Abstract
Liang Xiao1,2,*, Bo Yang1,4,*, Xiaoyu Liu1, Yan Luo1, Qiongmei Ji1, Zhong Wen3, Zhigang Liu1, Ping-Chang Yang1
1The Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen 518060, China
2BGI Shenzhen, Shenzhen 518000, China
3Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China
4Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
*These authors have contributed equally to this work
Correspondence to:
Ping-Chang Yang, email: [email protected]
Zhigang Liu, email: [email protected]
Zhong Wen, email: [email protected]
Keywords: food allergy, intestine, microbiota, 16S rRNA, animal model
Received: August 08, 2016 Accepted: October 04, 2016 Published: October 21, 2016
ABSTRACT
Food allergy (FA) is an adverse immune response to certain innocent food. It is estimated about 2% to 6% of the general population suffer from FA. Symptoms of a food allergic reaction may involve the gastrointestinal tract or/and other organs. The gut microbiota plays a critical role in diet-induced health problems. Whether the changes in the composition of the intestinal microbiota regulate allergic responses to food remains poorly understood. Thus, we created an FA animal model, sequenced the V4-V5 regions of 16S rRNA genes to characterize the genera abundance of gut microbiota. The results showed that mice under FA condition showed different gut bacterial structures. Diverse distribution of the bacterial species was identified between FA and control groups. FA altered the components of intestinal Microbiota in mice. The dysbiosis of the gut metagenome correlated with the development of the FA.
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